A Study on Liver-Injury of SD Rats with Autologous Orthotopic Liver Transplantation by Bile Duct Angiography using Meglumine Diatrizoate and Omnipaque

Objective: To investigate the liver-injury of the SD rats in autologus orthotopic liver transplatation using Meglumine Diatrizoate and Omnipaque. Methods: The portal veins of 60 healthy SD rats were perfused to set up orthotopic liver autotransplatation models. Then the external drainages of rats’ bile duct were built up by bile duct cannula. The ALT and AST in blood serum were detected 24h and 48h after the operation respectively using the recommended dose of Meglumine Diatrizoate, Omnipaque and normal saline. The blank trial group and simlpe operation group were the control group.Results: The ALT in the SD rats with orthotopic liver autotransplatation are higher than that in the blank trial group there is no significant difference between the blank trial group with the normal saline and simple operation group. Although there is significant difference between the blank trial group with the Meglumine Diatrizoate and the Omnipaque group. The bile duct angiography were performed after 24h and 48h. The AST in the SD rats with orthotopic liver autotransplatation are higher than that in the blank trial group;there is no significant difference between the blank trial group and simple operation group. But there is significant difference between the blank trial group with the Meglumine Diatrizoate and the Omnipaque group; there is significant difference after 24h between the blank trial group and the normal saline group; however there is no significant difference after 48h between the blank trial group and the normal saline group.Conclusion: Meglumine Diatrizoate and Omnipaque can damage the liver of SD rats in autologous orthotopic liver transplantation during bile duct angiography. The damage from Meglumine Diatrizoate is much more than Omnipaque.